Where is spectrophotometry applied?

Spectrophotometry is an analytical technique that allows determining the concentration of a compound in solution. It is based on the principle that molecules absorb electromagnetic radiation and in turn that the amount of light absorbed depends linearly on the concentration. A spectrophotometer is used to measure the amount of light absorbed by a solution.

Advantages of spectrophotometer for detecting impurities in food

Food that reaches the consumer has already gone through a journey from the producer, packaging, distribution and placing on the shelf. During this process, food is exposed to foreign agents. These impurities are called contaminants, which can become harmful to people’s health, or damage the product causing loss of money. Its origin is diverse and contamination can occur at any stage.

How is the Spectrophotometer used in the Cosmetic Industry?

The quality designation in the cosmetic industry, is composed of different variables. Among them, color is a very important element, since it allows to improve the appearance of a product or exert a specific effect on human skin, for example, makeup.

What is a UV-VIS spectrophotometer?

A spectrophotometer is a piece of equipment used to measure the absorbance of a sample, as a function of the wavelength of electromagnetic radiation. There are several types of spectrophotometers. These are grouped according to the type of sample analyzed; There are atomic absorption and molecular absorption (commonly known as a UV-VIS spectrophotometer).

What technology uses a spectrophotometer?

A spectrophotometer is a laboratory equipment used to measure the absorbance of a sample, as a function of the wavelength of an electromagnetic radiation, and thus know the concentration of substances in a solution. In general, this equipment consists of 4 main parts: a source, a monochromator, a beam splitter, a sample area and a detector. It also has optical elements such as lenses or mirrors, which transmit light throughout the entire equipment.

What is a Beam of Light in a Spectrophotometer?

The light beam of a spectrophotometer meets the projection of monochromatic luminescence to a sample, which is used to measure the amount of emission absorbed by said sample, also allows giving information on the state of the substance that integrates the sample, and indirectly tell the user what amount of the substance is of interest to him. 

Benchtop spectrophotometer to measure color in food

The importance of color as a physical characteristic and quality in food have a relevant role related to food additives. Consumers prefer attractive-looking products, and color is the first thing they evaluate. Usually, dyes are used to highlight the natural color of foods or to return the color lost in chemical processes carried out for their preservation. However, color in foods is not just an aesthetic attribute.

How is the color of a sample measured using the Colorimeter?

The colorimeter is an instrument used to determine the color of a sample. It is based on a photometer, which measures the intensity of light at a specific wavelength and compares the result with the intensity of a reference light at the same wavelength. The comparison is made by using an optical density scale or calibration curve.

Identification of anions by colorimeter

Anions are negatively charged ions, are in solution and are attracted to the anode, or positive electrode. To identify anions in solution, we can use a colorimeter. A colorimeter is an instrument that uses light to measure the concentration of a solution. Light passes through the solution and its absorbance is measured, it is then related to the concentration of the solution.